Suitable for Affinity Purification and Immunoprecipitation. Other applications not tested.

Antibody Purification: A mouse monoclonal IgG was purified from hybridoma supernatant fraction using a 12ml column of Protein G (Agarose), Fast Flow and a Waters 650E Advanced Protein Purification System. Elution Profile and Analysis: Mouse IgG was purified from 2L of hybridoma supernatant fraction using Protein G Agarose, Fast Flow. The column eluent was monitored with an UV detector and recorded at 280nm. Immunoprecipitation: 20ul of a 50% slurry (10ul packed beads) was used to capture a mouse IgG Immunoprecipitation complex. Immunoprecipitation of Phosphotyrosine containing proteins contained in EGFstimulated A431 cell lysate using anti-phosphotyrosine, (4ug) in conjunction with 20ul (50% slurry) Protein G Agarose, fast flow followed by immunoblot analysis using antiphosphotyrosine. Blot was developed using an HRP-conjugated secondary antibody and the ECL detection system.

May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Supplied as 10ml packed beads, containing 2mg/ml Protein G suspended as a 50% slurry in distilled water containing 20% ethanol for a final volume of 20ml. Binding capacity of 20mg human IgG/ml agarose. Prior to use, wash the agarose beads with an appropriate buffer to remove the ethanol.