Podoplanin

Clone Type

Monoclonal

Host

Mouse

Source

Human

Isotype

IgG1

Clone Number

6A457 (4D5aE5E6)

Grade

Affinity Purified

Applications

IHC

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Podoplanin is absent on blood endothelium and therefore this product is readily able to distinguish between lymphatic vessels and blood vasculature. Although the specific function of this protein is undetermined, Podoplanin is reported to be a reliable indicator of tumor lymphangiogenesis.

Applications

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution

Immunohistochemistry (Frozen and paraffin): 0.75-2ug/ml Note: Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Use sodium citrate buffer, pH 6.0. Optimal dilution determined by the researcher.

Recommended Secondary Reagents

I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo

Recommended Negative Control

I1904-78R: IgG1 Murine Negative Control

Recommended Positive Control Tissue

Normal lung

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

ORF Human podoplanin ectodomaine expressed in E. coli.

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Purity

Purified by Protein G affinity chromatography from tissue culture supernatant.

Specificity

Recognizes Human Podoplanin (gp36), a 36kD type I cell membrane-associated glycoprotein, with a broad spectrum of distribution, being strongly expressed on the lymphatic endothelium found in lung, placenta and skeletal muscle and to a lesser extent in brain, kidney and liver.

References

1. Matsui, K. et al. (2003). J. Am. Soc. Nephrol. 14: 1981-1989.2. Dadras, S. S. et al. (2003). Am. J. Pathol. 162: 1951-1960.3. Zimmer, G. et al. (1999). Biochem J. 341: 277-284.4. Schacht, V. et al. (2003). EMBO J. 22: 3546-3556.5. Kato, Y. et al. (2003). Molecular Identification of Aggrus/T1alpha as a Platelet Aggregation-inducing Factor Expressed in Colorectal Tumors. J. Biol Chem 278: 51599-51605.

USBio References

No references available

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P9104-91 Mouse Anti-Podoplanin (PDPN, Aggrus, Glycoprotein 36kD, Glycoprotein 36, gp36, GP38, HT1A-1, hT1alpha1, hT1alpha2, Lung Type-I Cell Membrane-associated Glycoprotein Isoform a, OTS8, OTS-8, PA2.26 Antigen, T1 alpha, T1A, TIA2)

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