MaxLight™ 550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
RSV is so named from the formation of syncytia, or multinucleated masses of fused cells. Two virally specified surface glycoproteins are prominent in RSV. The large glycoprotein is designated G and the other fusion protein is designated F. In addition, two distinct groups may be present in RSV, Group A and/or Group B.
Applications
Suitable for use in Immunofluorescence, ELISA and Neutralization. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
RSV, types A and B fusion protein
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Purity
Purified by Protein A affinity chromatography from ascites fluid or culture|medium.
Specificity
Recognizes the fusion protein of RSV, types A,B