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R2031-71D Rabbit Anti-RIG-1 (Retinoic Acid Inducible Gene 1 Protein, RIG-I, Probable ATP-Dependent RNA Helicase DDX58, Dead Box Protein 58, DDX58)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q96C10; O95786
Isotype
IgG
Grade
Highly Purified
Applications
IHC WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

RIG-I is a receptor found in the cytoplasm that recognizes viral RNA from replicating viruses in infected cells. Upon activation with intracellular RNA, a cascade is triggered resulting in the activation of NF-kappa-B, and the eventual induction of antiviral cytokines such as IFN-beta and RANTES. RIG-I is essential for the production of interferons in response to RNA viruses and as such play a key role in innate immunity.

Applications
Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended Dilution
Immunohistochemistry (paraffin): 5ug/ml detects a band at ~84kD in C2C12 cell lysate. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate, pH 6, is recommended. Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human heart
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human GST-tagged RIG-1 protein.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by DEAE chromatography.
Specificity
Recognizes human RIG-I. Species Crossreactivity: mouse and rat
References
1. Akira S, et al (2006) Pathogen recognition and innate immunity 124:783-801. 2. Yoneyama M et al (2004) The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses. Nat. Immunol.; 5:730-737. 3. Sharma S, et al (2003) Triggering the interferon antiviral response through an IKK-related pathway Science 300:1148-51. 4. Alexopoulou L, et al (2001) Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3 Nature 413:732-8
USBio References
No references available
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