Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM.
Applications
Suitable for use in FLISA, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Fluorescein/IgG protein molar ratio (F/P)
~1.6
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Immunogen
Purified free monkey secretory component isolated from pooled milk.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Labled with Fluorescein isothiocyanate (FITC). Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Purity
Purified by DEAE chromatography.
Specificity
Recognizes monkey secretory component. Reacts with both bound secretory component (secretory IgA) and with the free SC present in monkey secretions. In immunoelectrophoresis against monkey milk, using a high electroendosmosis agar plate, free SC is precipitated in the alph-2 region. Does not react with other molecular forms of IgA, or with any other secretory or plasma protein. Precipitation reactions have been observed with free and bound secretory component in serum of other old-world monkeys, including Cercopithecus, Cynomolgus and Baboon.