Suitable for use in ELISA, Western Blot and Neutralization. Other applications not tested.

Western Blot: 0.5ug/ml using SH‑SY5Y human neuroblastoma cell line and human lymph node Neutralization: 1.5-7.5ug/ml (ND50) in the presence of 5ug/ml recombinant human Siglec-1/CD169 Fc Chimera. Optimal dilutions to be determined by the researcher.

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recombinant protein corresponding to Ser20-Gln1641 of human Siglec-1 expressed in NS0 cells

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.

Purified by immunoaffinity chromatography.|Endotoxin: ≤0.1EU/1mg (LAL)

Recognizes human Siglec-1 in direct ELISA and Western Blot. In direct ELISA, this antibody shows ~20% cross-reactivity with rmSiglec-1 and <1% cross-reactivity with rhSiglec-2.