Siglec 7 is a type I transmembrane glycoprotein and member of the Siglec (sialic acid binding Ig-like lectin) family, originally identified as an inhibitory NK cell receptor (NKR) and negative regulator of NK activation, attributed to ITIM recruitment of SHP-1 phosphatase. Siglec 7 is expressed predominantly by natural killer cells (NK) and to a lesser extent by monocytes and granulocytes and, like Siglec-5 (CD170), has been shown to bind to sialylated ligands of targets through recognition of sialic acid in both the alpha-2,3- and alpha-2,6- glycosidic linkage.

Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Flow Cytometry: 1:100-1:200; 10ul labels 1x10e6 cells in 100ul. Immunohistochemistry (Frozen): 1:100 Optimal dilutions to be determined by the researcher.

Frozen tonsil

I1904-78R: IgG1 Murine Negative Control

X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Monocyte-derived dendritic cells.

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.

Purified by Protein G affinity chromatography from tissue culture supernatant.

Recognizes human Siglec 7.