USBio Logo

S1014-52L Rabbit Anti-Smad2 (SMAD Family Member 2, SMAD 2, SMAD-2, hMAD-2, hSMAD2, JV18-1, JV18, MAD-related Protein 2, MADR2, Mothers Against Decapentaplegic Homolog 2, Mothers Against DPP Homolog 2, MAD Homolog 2, MADH2)

Specifications
References
Clone Type
Monoclonal
Host
Rabbit
Source
Mouse
Swiss Prot
Q15796
Isotype
IgG
Clone Number
11C1070(D43B4)
Grade
Purified
Applications
ChIP FC IF IP WB
Crossreactivity
Hu Mk Mo Rt
Gene ID
4087
Shipping Temp
Blue Ice
Storage Temp
-20°C

Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-B signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8).

Applications
Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Immunofluorescence (IF-IC): 1:50 Flow Cytometry: 1:200 Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Chromatin Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues near the N-terminus of mouse Smad2.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity
Purified
Specificity
Recognizes endogenous levels of total Smad2. Species Crossreactivity: human, rat and monkey.
References
1) Heldin, C.H. et al. (1997) Nature 390, 465-471. (2) Attisano, L. and Wrana, J.L. (1998) Curr. Opin. Cell Biol. 10, 188-194. (3) Derynck, R. et al. (1998) Cell 95, 737-740. (4) Massague, J. (1998) Annu. Rev. Biochem. 67, 753-791. (5) Whitman, M. (1998) Genes Dev. 12, 2445-2462. (6) Wu, G. et al. (2000) Science 287, 92-97. (7) Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-1647.
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved