Suitable for use in ELISA, Western Blot, Immunoprecipitation. Other applications not tested.

Peptide ELISA: 1:32,000 Western Blot: 0.5-2ug/ml, ~60-65kD band seen in mouse NIH-3T3 cell lysate Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Corresponding peptide is available for Peptide Blocking studies. See S1014-54D-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.

Peptide with sequence C-HTMPIADPQPLD, from the C-terminus of the protein sequence according to NP_005350.1. Species sequence homology: canine, porcine and bovine

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

Purified by immunoaffinity chromatography.

Recognizes human SMAD4. Species Crossreactivity: mouse and rat