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S1040-01N Rabbit Anti-SOCS 1, NT (Suppressor of Cytokine Signalling 1, Janus kinase (JAK) binding protein, STAT-induced STAT inhibitor, SSI-1)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
CAB92528
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Description
The Suppressor of cytokine signaling (SOCS) and cytokine-inducible SH2 proteins are a family of intracellular proteins which regulate the immune cell responses to cytokines (1). SOCS1 acts to suppress dendritic cell (DC) as well as T cell hyperactivation following cytokine signaling by inhibiting JAK tyrosine kinase, a kinase necessary for type I and II cytokine receptors to initiate signaling, by directly binding to the catalytic domain of the kinase (2). SOCS1 also possesses E3 ubiquitin protein ligase activity that results in the polyubiquitination of its target proteins and subsequent degradation by the proteosome (3). It is through this method that SOCS1 negatively regulates signaling by Toll-like receptors TLR2 and TLR4 by mediating the degradation of the TLR signaling adaptor protein TIRAP (4).
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug’ml Optimal dilutions to be determined by the researcher.
Positive Control
Human cell lysate
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide, 17aa, near the amino terminus of human SOCS1 (UniProt # CAB92528).
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SOCS1. Species Crossreactivity: mouse and rat
References
1. Rakesh K and Agrawal DK. Controlling cytokine signaling by |constitutive inhibitors. Biochem. Pharm. 2005; 70:649-57. |2. O’Shea JJ, Gadina M, and Schreiber RD. Cytokine signaling |in 2002: new surprises in the Jak/Stat pathway. Cell 2002; |109:S121-31. |3. Kile BT, Schulman BA, Alexander WS, et al. The SOCS box: |a tale of destruction and degradation. Trends Biochem. Sci. |2002; 27:235-41. |4. Mansell A, Smith R, Doyle SL, et al. Suppressor of cytokine |signaling 1 negatively mediates Toll-like receptor signaling by |mediating Mal degradation. Nat. Immunol. 2006; 7:148-55. |(WD0306)
USBio References
No references available
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