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S6610-10-PE Mouse Anti-Stage Specific Embryonic Antigen 4 (SSEA4) (PE)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
PE
Isotype
IgG3
Clone Number
5C1 (MC-813-70)
Grade
Affinity Purified
Applications
FLISA IF IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

Expression of SSEA4 is down-regulated following differentiation of human tetracarcinoma stem (EC) cells. In contrast, the differentiation of murine tetracarcinoma stem (EC) cells and embryonic stem (ES) cells may be accompanied by an increase in SSEA4 expression.

Applications
Suitable for use in FLISA, Immunohistochemistry, Flow Cytometry and Immunofluorescence. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Recommended Positive Control
Pluripotent human embryonic stem cells.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Human embryonal carcinoma cell line 2102Ep.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes Stage-specific embryonic antigen 4 (SSEA4) that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. The differentiation of murine EC. and ES cells may be accompanied by an increase in SSEA-4. Species Crossreactivity: mouse.
References
1. Kannagi, R., et al., EMBO Journal 2: 2355-2361 (1983). 2. Fenderson, B.A., et al., Dev. Biol. 122: 21-34 (1987). 3. Shamblott, M.J., et al., PNAS 95: 13,726-13,731 (1998). 4. Thomson, J.A., et al., Science 282: 1145-1147 (1998). 5. Reubinoff, B.E., et al., Nature Biotechnology 18: 399-404 (2000). 6. Henderson, J.K., et al., Stem Cells 20: 329-337 (2002).
USBio References
No references available
Conjugates
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