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S6610-11 Mouse Anti-Stage Specific Embryonic Antigen 4 (SSEA4)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG3
Clone Number
8H218
Grade
Affinity Purified
Applications
FC IC
Crossreactivity
Hu Mo
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.

Applications
Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 0.25ug/10e6 cells. Using NTera-2 human testicular embryonic carcinoma cell line Immunocytochemistry: 8-25ug/ml using immersion fixed BG01V human embryonic stem cells cultured on irradiated mouse embryonic fibroblasts Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder and reconstituted products must be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
2102Ep human embryonal carcinoma cell line
Form
Supplied as a lyophilized powder from 50mM MES, pH 6.5, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant
Specificity
Recognizes a carbohydrate epitope of human SSEA-4. Species Crossreactivity: mouse
References
1. Shevinsky, L.H. et al., 1982, Cell 30: 697-705. 2. Kannagi, R. et al., 1983, EMBO J. 2: 2355-2361. 3. Thomson, J.A. and J.S. Odorico, 2000, Trends Biotechnol. 18: 53-57. 4. Draper, J.S. et al., 2002, J. Anat. 200: 249-258.
USBio References
No references available
Conjugates
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