Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:32000 Western Blot: 0.2-0.5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-DHSESGEVRFHSVEP of human STAT4.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human STAT4. Species sequence homology: Canine and porcine.
References
1. Good SR, et al. Temporal induction pattern of STAT4 target genes defines potential for Th1 lineage-specific programming. Journal of immunology (Baltimore, Md. : 1950) 2009 Sep 183 (6): 3839-47.
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