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S8401-02D Rabbit Anti-Surfactant Protein B (SP-B)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Bo Hu Mo Po
Shipping Temp
Blue Ice
Storage Temp
-20°C

There are four surfactant-specific proteins, designated surfactant protein A (SP-A), SP-B, SP-C and SP-D respectively. SP-A and SP-D are hydrophilic surfactant proteins and are members of the collectin family. SP-B and SP-C are hydrophobic surfactant proteins and may be the most appropriate indicators for the evolutionary origin of surfactant. SP-B is synthesized by the alveolar type II epithelial cells as a 40-42 kD precursor that is subsequently proteolytically processed to 7.8-8 kD. SP-B enhances the spreading and stability of surfactant phospholipids in the alveolus. SP-B is essential for air-breathing in mammals and is therefore largely conserved. SP-B can interact with both phospholipid head groups and fatty chains and is particularly active in enhancing surface active behavior in endogenous and exogenous lung surfactants. Even low SP-B contents had measurable effects in increasing the adsorption, dynamic surface tension lowering, and/or film respreading of DPPC, mixed synthetic lipids, and column-purified lung surfactant phospholipids. Deficiency of SP-B and other surfactant components is associated with respiratory distress syndrome (RDS) in premature infants and adults with respiratory distress syndrome (ARDS).

Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:50. Recognizes proteins of 42, 25 and 18kD under non-reducing conditions. The 18kD form is reduced to 7.7-8kD in the presence of beta-mercaptoethanol. Immunohistochemistry (Frozen, paraffin): 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic polypeptide corresponding to 60aa from human Surfactant Protein B (SP-B).
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human Surfactant Protein B (SP-B). Species Crossreactivity: mouse, bovine and porcine
References
1. Whitsett, J., et al., Immunologic identification of a pulmonary surfactant-assosiated protein of molecular weight = 6000 daltons. Pediatr. Res. 20: 744 (1986). 2. Weaver, T., et al., Identification of surfactant proteolipid SP-B in human surfactant and fetal lung. J. Appl. Physiol. 65: 982 (1988). 3. Gregory, T., el al., Surfactant chemical composition and biophysical activity in acute respiratory distress syndrome. J. Clin. Invest. 88: 1976 (1991). 4. Clark, J., et al., Targeted disruption of the surfactant protein B gene disrupts surfactant homeostasis, causing respiratory failure in newborn mice. PNAS 92: 7794 (1995). 5. Nogee, L., et al., Allelic heterogeneity in hereditary surfactant protein B (SP-B) deficiency. Am. J. Respir. Crit. Care Med. 161: 973 (2000).
USBio References
No references available
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