USBio Logo

S8500-01M Mouse Anti-Survivin (Apoptosis Inhibitor 4, Apoptosis Inhibitor-4, API4, Apoptosis Inhibitor Survivin, Baculoviral IAP Repeat-containing 5, BIRC5, BIRC-5, EPR1, EPR-1, IAP4, SVV, TIAP)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
O15392
Isotype
IgG1,k
Clone Number
9E763
Grade
Affinity Purified
Applications
E FC IC IF IHC IP WB
Crossreactivity
Hm Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Regulated inhibition of programmed cell death (apoptosis) preserves normal homeostasis and tissue and organ morphogenesis. Aberrations in this process contribute to human diseases and cancer by abnormally prolonging cell viability. Recently, several apoptosis inhibitors related to the baculovirus iap gene have been found in various species, including human. IAP proteins contain one/three Cys/His baculovirus IAP repeats plus a c-terminus RING finger and are thought to block an evolutionary conserved step in apoptosis. Survivin encodes a structurally unique inhibitor of apoptosis (IAP). Survivin expression is turned off during fetal development and is not found in non-neoplastic adult human tissues. Survivin becomes abundantly re-expressed in transformed cells and in all of the most common cancers of lung, colon, pancreas, breast and prostate in vivo. Survivin appears to be situated at the crossroads of cell death and cell division, governing a checkpoint involved in cytokinesis while also suppressing apoptosis.Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affect regulation of neural cell proliferative responses after brain injury.

Applications
Suitable for use in ELISA, Flow Cytometry, Immunofluorescence/Immunocytochemistry, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (Paraffin-embedded sections): 20ug/ml Requires HIER using sodium citrate buffer pH 6.0 prior to staining. Immunofluorescence (IC): 1:10-1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full-length recombinant protein corresponding to human survivin
Form
Supplied as a liquid in PBS, 0.02% sodium azide
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes the nuclear form of human survivin at ~16.5kD in Western Blot. The epitope recognized is between amino acids 3-19. Species Crossreactivity: hamster
References
1. G. Ambrosini et al. Induction of Apoptosis and Inhibition of Cell Proliferation by survivin Gene Targeting. JBC, 273(18): 11177-11182, 1998. 2. F. Li et al. Pleiotropic cell-division defects and apoptosis induced by interference with survivin function. Nature Cell Biology, 1(8): 461-466, 1999. 3. D. Grossman et al. Expression and targeting of the apoptosis inhibitor, survivin, in human melanoma. J. of Invest Dermatology, 113(6): 1076-81, 1999. 4. F. Li, et al. Control of apoptosis and mitotic spindle checkpoints by survivin. Nature. 396: 580-584, 1998. 5. D. Grossman, et al. Expression and Targeting of the Apoptosis Inhibitor, Survivin, in Human Melanoma. J. Invest. Dermatology. 113: 1076-1081, 1999. 6. C. Adida, et al. Prognostic significance of survivin expression in diffuse large B-cell lymphomas. Blood. 96(5): 1921-1925, 2000.
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved