Syndecans are heparan sulfate proteoglycans that consist of a hydrophobic membrane-spanning domain, a short cytoplasmic domain, and an extracellular domain. Syndecans contain a small core protein with distinct functional domains to which multiple glycosaminoglycan chains are linked, predominantly of the heparan sulfate variety. Syndecans are involved in cell adhesion, tissue morphogenesis and differentiation and regulation of cell responsiveness to soluble growth-regulatory compounds. These functions are attributed primarily to the glycan moieties. Syndecans also interact with growth factors such as fibroblast growth factor and epidermal growth factor. Syndecan 2 was originally cloned from embryonic fibroblasts. Syndecan 2 is expressed in epithelial-mesenchymal interfaces, and prechondrogenic and preosteogenic mesenchymal condensations, which are areas of high morphogenetic activity. Syndecan 2 is involved in the mitogenic activity and signaling of Granulocyte-Macrophage Colony-stimulating factor in osteoblasts, and has been shown to have a regulatory role in matrix assembly. Increased Syndecan 2 expression is important for colon carcinoma behavior, and Syndecan 2 regulates tumorgenic activity by regulating adhesion and proliferation in colon carcinoma cells.

Suitable for use in Western Blot and FLISA. Other applications have not been tested.

Western Blot: 1-3ug/ml FLISA: 0.1-1ug/ml Optimal dilutions to be determined by the researcher.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Synthetic peptide corresponding to the internal region of human syndecan-2 (fibroglycan).

Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490.

Purified by immunoaffinity chromatography.

Recognizes the unglycanated form of human syndecan-2 (fibroglycan). On Western Blots, it identifies a single band at ~22kD. An additional band at ~40kD may also be observed which may represent a glycanated form. Reactivity has been confirmed with human A-549 lung carcinoma, HEP-G2 hepatocellular carcinoma, HT-29 colorectal adenocarcinoma, K-562 chronic myelogenous leukemia-bone marrow, TF-1 erythroleukemia-bone marrow and U-2 OS osteosarcoma cell lysates.