TACC2, also known as transforming acidic coiled-coil-containing protein 2, belongs to the transforming acidic coiled-coil-containing (TACC) protein family. This group of proteins is defined by its highly conserved coiled-coil domain. This domain is necessary for the assignment of the centrosome-spindle apparatus during cell cycling. There are three proteins belonging to the TACC family; TACC1, TACC2, and TACC3. TACC2 has been reported as playing a necessary role in cultured cell microtubule stabilization. It has also been shown that the binding of TACC2 by T antigen can cause chromosomal instability and mitotic defects (Tei, 2009). Research into the over expression of TACC2 strongly suggests a potential function as a tumor suppressor (Tei, 2009).

Suitable for use in Western Blot. Other applications have not been tested.

Western Blot: detects TACC2 on 10ug of mouse testis tissue lysate Optimal dilutions to be determined by the researcher.

I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Linear peptide corresponding to the mature chain of mouse TACC2, conjugated to KLH. Species Sequence Homology: rat

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.

Purified by affinity chromatography.

Recognizes mouse TACC2, Mr ~65kD. TACC2 has a reported Mr ~73kD in HeLa cell lysates. The immunizing peptide inhibited antibody recognition of the Mr ~65kD band in a competition experiment. Species Crossreactivity: human