Human TARC is a recently discovered protein belonging to the beta-chemokine (C-C) family of cytokines, secreted by activated monocytes, thymic and lymph node dendritic cells. It is chemotactic towards activated T cells (Th2 subset) which express express CCR4 but ineffective on monocytes. Human TARC is an 8.0kD protein containing 71 amino acid residues.
Applications
Suitable for use in Neutralization, ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Neutralization: 2-3ug/ml will yield one-half maximal inhibition [ND50] of the biological activity of human TARC (100ng/ml). ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution In conjunction with biotylated anti-human TARC (T1020-05A) as a detection antibody, allows the detection of at least 2000-4000pg/well of recombinant human IL-22. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human TARC is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to human TARC expressed in E. coli.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human TARC.
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