EC=2.7.7.31; Nucleosidetriphosphate DNA; Deoxynucleotidylexotransferase; Terminal Deoxyribonucleotidyltransferase, TdT, Deoxynucleotidyltransferase Terminal DNA; DNTT, Terminal Addition Enzyme; Terminal Transferase
DNA nucleotidylexotransferase (DNTT) belongs to the DNA polymerase type-X family. DNTT catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to aa472-486, DNHALYDKTKRIFLK to human DNA nucleotidylexotransferase.
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitute with 100ul of sterile dH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human DNTT at 58kD.
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