MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
TLR11 is principally expressed in macrophages and liver, and epithelial cells of the kidney and bladder. Mouse TLR11 is reported to recognise uropathogenic bacteria, and to activate appropriate host defense genes as part of the innate immune response to microbial infection.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-1ug/ml Immunohistochemistry (frozen): 10ug/ml Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
Goat Anti Rabbit IgG (H&L) HRP Sheep Anti Rabbit IgG Biotin Goat Anti Rabbit IgG (Fc) FITC, HRP Goat Anti Rabbit IgG (H&L) HRP Sheep Anti Rabbit IgG FITC Sheep Anti Rabbit IgG RPE Sheep Anti Rabbit IgG DyLight™488, DyLight™549, DyLight™649 Sheep Anti Rabbit IgG HRP
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to a 15 aa sequence near the carboxy terminus of mouse TLR11.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by Ion Exchange chromatography.
Specificity
Specific for the C-terminal of mouse Toll-like receptor 11 (TLR11).