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T8250-16E-ML650 Mouse Anti-Transforming Growth Factor beta 1 (TGFb1, TGF-beta-1, TGFB1, TGFB) (MaxLight 650)

Specifications
References
Brand
MaxLight™
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P01137
Conjugate
MaxLight™650
Isotype
IgG1
Clone Number
10F521 (TB21)
Grade
Affinity Purified
Applications
FC FLISA IHC WB
Crossreactivity
Hu Mo Rb Rt Sh
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

TGFb1 (Transforming Growth Factor-beta1) is a family of multifunctional 25kD proteins. TGFb1 was originally identified for its ability to induce the growth of normal rodent fibroblasts in soft agar. It is now known that TGFb1 is a potent growth inhibitor of many normal and transformed cell lines. It regulates normal cell growth, development and tissue remodeling following injury. TGFb1 is produced as latent high molecular weight complexes. The activation of latent TGFb1 is an important step in the regulation of its action.
Applications
Suitable for use in FLISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Flow Cytometry: Membrane permeabilization is required. Immunohistochemistry: Frozen sections Immunohistochemistry (Paraffin): Has been successfully used on FFPE tissue without pre-treatment. Can use citrate, pH 6, EDTA, pH 8, or pepsin mediated antigen retrival. Optimal dilutions to be determined by the researcher.
Recommended Negative Control
I1904-78R: IgG1 Murine Negative Control
Immunohistochemistry Postivie Control
Human Breat Carcinoma
Hybridoma
SP2/0-Ag 14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Human Transforming Growth Factor beta 1 from platelets.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human platelet-derived and recombinant Transforming Growth Factor beta 1 in ELISA. Demonstrates neutralizing activity against TGF-beta1 in cell proliferation assays. Demonstrated to react with dimeric (25kD) or monomeric (12.5kD) molecules of natural TGFb1 under non-reducing and reducing conditions respectively. Species Crossreactivity: rat, sheep, mustelid, rabbit, mouse, mink
References
1. Lehr, E.J.et al. (2010) Decellularization reduces immunogenicity of sheep pulmonary artery vascular patches. J Thorac Cardiovasc Surg. Jul 14. [Epub ahead of print] 2. Westermann, D. et al. (2011) Circ Heart Fail. 4: 44­52. 3. Brown, H. et al. (1999) J Infect Dis. 180: 1742­6. 4. Helske, S. et al. (2006) Eur Heart J. 27: 1495­504. 5. Hsiao, Y.W. et al. (2004) J Immunol. 172: 1508­14. 6. Kingston, P.A. et al. (2003) Circulation. 108: 2819­25. 7. Lavaud, S. et al. (2001) Am J Physiol Renal Physiol. 280: F683­94. 8. Sheu, B.C. et al. (2001) J Immunol. 167: 2972­8. 9. Hopkinson, A. et al. (2006) Invest Ophthalmol Vis Sci. 47: 4316­22. 10. Sumpter, T.L. et al. (2007) J Leukoc Biol. 83: 708­17. 11. Braun, N. et al. (2011) Perit Dial Int. 31: 291­ 300. 12. Shi, W.K. et al. (1993) [Biological characterization of a monoclonal antibody TB 21 against human transforming growth factor­beta 1]. Shi Yan Sheng Wu Xue Bao. 26: 141­50.
USBio References
No references available
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