MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
Troponin I is 1 of 3 subunits that constitute the troponin complex which plays an important role in the regulation of skeletal and cardiac muscle contraction. It is presented in cardiac tissue by a single isoform with a molecular weight of 29kD and is known as a specific marker for cardiac tissue damage.
Applications
Suitable for use in FLISA and Western Blot. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Free human cardiac troponin I
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes cardiac and skeletal human troponin I. It is specific for an epitope between aa186-192 of cardiac troponin I. |Species Crossreactivity: Mouse, feline, Rabbit, canine, porcine, Rat, Goat, Bovine and Fish.