As major neutral proteases present in the mast cells, tryptases function as mediators in the pathogenesis of asthema and other allergic and inflammatory disorders. Tryptase, beta2 (TPSB2) consists of a signal peptide (residues 1 to 21), a pro peptide (residues 22 to 31) and a mature chain (residues 32 to 275). The amino acid sequence of mouse TPSB2 is 88%, 78%, 76%, and 73% identical to that of mouse, human (TPSB2 and TPSAB1), chimpanzee, and canine.
Applications
Suitable for use in Immunoprecipitation, ELISA and Neutralization. Other applications not tested.
Recommended Dilutions
Immunoprecipitation: 25ug/ml immunoprecipitates recombinant mouse Tryptase, beta 2 from conditioned cell culture medium. Neutralization: Measured by its ability to neutralize Recombinant Mouse Mast Cell Protease6/Mcpt6 (1ug/ml) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10uM). The Neutralization Dose (ND50) is typically 3ug/ml. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Ile32-Ser276 from mouse Tryptase, beta2 expressed in NS0 cells.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Purity
Purified by Protein A or G chromatography from hybridoma culture supernatant
Specificity
Recognizes mouse Tryptase, beta2.
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