Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55kD. Microtubules are large structures composed of alternating alpha and beta subunits. Compounds that block microtubule formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C-terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin.

Suitable for use in RIA, FLISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Western Blot: detects tubulin on 10ug of A431 lysates. A431 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with T9154-04 (1:500). Proteins were visualizes using a goat anti-rat secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Yeast tubulin

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.

Purified by Protein A affinity chromatography.

Recognizes the alpha subunit of tubulin, specificity binding to so called Tyr-Tubulin at ~55kD, which is produced by post translational modification of tubulin. Species crossreactivity: human, mouse and rat.