Human beta-Tubulin 3 is a 50.432kD structural protein (450aa) expressed in neurons of the PNS and CNS. It contributes to microtubule stability in neuronal cell bodies and axons, and plays a role in axonal transport..
Applications
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:2000-1:5000 Immunohistochemistry/Immunocytochemistry: 1:1000-1:2000 detects Tubulin, beta 3 in 2% paraformaldehyde-fixed tissues or cells. Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1906-10: IgY, Chicken (HRP) Rb x Ch I1906-12: IgY, Chicken (FITC) Rb x Ch I1906-14: IgY, Chicken (AP) Rb x Ch
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Three synthetic peptides corresponding to different regions of the beta-Tubulin 3, conjugated to KLH. Species Sequence Homology: human and rat; 100%.
Form
Supplied as a liquid in 10mM PBS, pH 7.2, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes beta-Tubulin 3. Species Crossreactivity: mouse.