Urocortin 3

Clone Type

Polyclonal

Host

Goat

Source

Human

Isotype

IgG

Grade

Affinity Purified

Applications

Crossreactivity

Accession #

NP_444277.2

Shipping Temp

Blue Ice

Storage Temp

-20°C

UCN3; urocortin 3 (stresscopin); MGC119002; SCP; SPC; UCNIII; stresscopin; urocortin 3; urocortin III

This gene is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is structurally related to the corticotropin-releasing factor (CRF) gene and the encoded product is an endogenous ligand for CRF type 2 receptors. In the brain it may be responsible for the effects of stress on appetite. In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin II.

Applications

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions

Peptide ELISA: 1:16,000 Western Blot: 1-2ug/ml. In transfected HEK293 transiently expressing full-length human UCN3 (myc and DYKDDDDK tagged), a band of ~19kD was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody.

Optimal dilutions to be determined by the researcher.

Peptide Blocking

U2575-03-P (sold separately): Urocortin 3, corresponding peptide; Peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration is 0.5mg/ml. Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20 minutes. Then incubate and develop the two blots in parallel.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Synthetic peptide corresponding to KPRQDTAKSPHRTK from the internal region of human Urocortin 3 (NP_444277.2)

Form

Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes Urocortin 3. Species sequence homology: human.

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U2575-03 Goat Anti-Urocortin 3 (UCN3, Stresscopin, MGC119002, SCP, SPC, UCNIII)

Clone Type

Host

Source

Isotype

Grade

Applications

Crossreactivity

Accession #

Shipping Temp

Storage Temp

UCN3; urocortin 3 (stresscopin); MGC119002; SCP; SPC; UCNIII; stresscopin; urocortin 3; urocortin III

References

USBio References

Conjugates

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