The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence.
Applications
Suitable for use in FLISA, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Hybridoma
SP2/0 Ag14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
A recombinant peptide corresponding to the PK tag fused to a class II-restricted T helper cell epitope.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes V5-TAG.
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