V5 is used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. The V5 epitope tag is derived from an epitope present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). Usually the V5 tag is used with all 14aa (GKPIPNPLLGLDST), although it has also been used with a shorter 9aa sequence (IPNPLLGLD).

Suitable for use in FLISA, Western Blot, Immunofluorescence and Immunoprecipitation. Other applications not tested.

Optimal dilutions to be determined by the researcher.

Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

A recombinant peptide corresponding to the PK tag fused to a class II-restricted T helper cell epitope.

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.

Purified by Protein G affinity chromatography from tissue culture supernatant.

Recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.