The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence.

The V5 epitope tag is a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.

Suitable for use in RIA, Immunofluorescence, Flow Cytometry, FLISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher.

SP2/0 Ag14 myeloma cells with spleen cells from Balb/c mice.

I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb xMo I1904-66C: IgG, F(ab’)2X-adsorbed (HRP) Pab Rb xMo

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Paramyxovirus SV5, Simian-Virus 5.

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.

Purified by Protein A affinity chromatography from tissue culture supernatant.

Recognizes the V5 epitope tag. Detects recombinant proteins tagged with the epitope, some of which include transmembrane and secreted proteins. Usually, a 14aa tag is added to the recombinant proteins, although a smaller epitope of 9aa (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined).