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V2121-87B-FITC Rabbit Anti-Vesicular GABA Transporter (solute carrier family 32, member 1, bA122O1.1, GABA and Glycine transporter, hVIAAT, Solute carrier family 32 member 1, Vesicular GABA transporter, Vesicular inhibitory amino acid transporter, VGAT, VIAAT) (FITC)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Rat
Swiss Prot
O35458
Conjugate
FITC
Isotype
IgG
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Rt
Accession #
NM_080552.2
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free

Vesicular GABA transporter (VGAT), the first vesicular amino acid transporter to be molecularly identified, differs in structure from previously cloned vesicular neurotransmitter transporters and defines a novel gene family. VGAT is highly concentrated in the nerve endings of GABAergic neurons in the brain and spinal cord but also in glycinergic nerve endings. In contrast, hippocampal mossy fiber boutons, which are glutamatergic but known to contain GABA, lack VGAT immunoreactivity. Although the great majority of nerve terminals containing GABA or glycine are immunopositive for VGAT, subpopulations of nerve endings rich in GABA or glycine appear to lack the protein. Additional vesicular transporters or alternative modes of release may therefore contribute to the inhibitory neurotransmission mediated by these two amino acids.

Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Positive Control
Rat brain lysate
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Vesicular GABA Transporter corresponding to the C-terminus, conjugated to KLH. (Acccession #NM_080552.2). Cellular Localization: Cytoplasmic vesicle membrane; Multi-pass membrane protein.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat VGAT at ~57kD. Species Sequence Homology: human, mouse and monkey.
References
1. Tabuchi, Katsuhiko, et al.. (2007) Science, 318: 71-6. 2. Chessler, SD et al.. (2002) Diabetes, 51:1763-1771 3. McIntire, S.L. et al.. (1997) Nature, 389:870-876.
USBio References
No references available
Conjugates
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