Vitellogenin (Vg) is an egg yolk precursor protein expressed in female fish, dormant in male fish and female insects, but in the presence of estrogenic endocrine disrupting chemicals (EDCs), males can express the Vg gene in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDCs.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:50-1:500 Western Blot: 1:50-1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Vitellogenin from whole body homogenate of 17b-estradiol treated three-spined stickleback.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 12.3mM sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes vitellogenin from stickleback (Gasterosteus aculeatus). |Species Crossreactivity: Rainbow trout (Oncorhynchus mykiss), tilapia (Oreochromis niloticus), sheepshead minnow (Cyprinodon variegatus), cod (Gadus morhua), turbot (Scophthalmus maximus) and ballan wrasse (Labrus bergylta).
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