The protein encoded by this gene is a cytoplasmic serine-threonine kinase that contains cysteine in place of the lysine found at the conserved ATP-binding location in subdomain II of protein kinases. Since this protein does have kinase activity, it is possible that another lysine in the kinase subdomain I can substitute for the missing conserved lysine. [provided by RefSeq]
Applications
Suitable for use in ELISA, Immunohistochemistry (paraffin), and Western Blot. Other applications not tested.
Recommended Dilution
Immunohistochemistry (paraffin): 1.5ug/ml Western Blot: 1:500-1:1,000 ELISA: 1ug/ml-3ng/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to a portion of amino acids within aa2118-2218 of human WNK2.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human WNK2.