Zinc is an essential nutrient for all organisms because of the many important roles this metal plays. Movement of zinc into and out of cells and subcellular organelles is mediated by zinc transporter proteins. In many organisms, zinc uptake is mediated by members of the ZIP family of metal ion transporters. In mammals, the Zip1, Zip2, Zip3, Zip4, Zip5, LIV- 1 (Zip6), KE4 (Zip7), and BIGM103 (Zip8) proteins have been implicated in zinc uptake in a variety of cell and tissue types.
Zip3 (SLC39A3) encodes a 314aa protein that has an N-terminal signal peptide. Overexpression of the mZIP3 homolog in HEK293 cells increased Zn uptake in similar fashion to mZIP1 and mZIP2; however, mZIP3 specificity for zinc could be less than reported for the two other Zip transporters. Zinc-depleted THP1 cells have downregulated Zip3 expression.
Applications
Suitable for use in Western Blot, ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1-10ug/ml (Chemiluminescence) ELISA: 1:100,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to 17aa of mouse ZIP3 (Accession #AAH05502), N-terminal (KLH).
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse ZIP3. Species sequence homology: rat (95%).
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