Zinc is an essential nutrient for all organisms because of the many important roles this metal plays. Movement of zinc into and out of cells and subcellular organelles is mediated by zinc transporter proteins. In many organisms, zinc uptake is mediated by members of the ZIP family of metal ion transporters. In mammals, the Zip1, Zip2, ZIP4, Zip4, Zip5, LIV-1 (Zip6), KE4 (Zip7), and BIGM103 (Zip8) proteins have been implicated in zinc uptake in a variety of cell and tissue types.
Zip5 (SLC39A5) is similar in sequence to the Zip4 protein, which is involved in the uptake of dietary zinc. Co-expression of Zip4 and Zip5 in the intestine led to the hypothesis that these proteins play overlapping roles in the uptake of dietary zinc across the apical membrane of intestinal enterocytes. mZip5 localizes specifically to the basolateral membrane of polarized Madin-Darby canine kidney cells. Zip5 plays a central role in controlling organismal zinc status.
Applications
Suitable for use in Western Blot, ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1-10ug/ml (Chemiluminescence) ELISA: 1:100,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to 17aa of mouse ZIP5 (Accession #Q9D856), N-terminal, Extracellular domain (KLH).
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse ZIP5.
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