ZO-1

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Conjugate

Biotin

Isotype

IgG

Grade

Affinity Purified

Applications

E IHC IP WB

Crossreactivity

Ca Gp Hu Mo Rt

Shipping Temp

Blue Ice

Storage Temp

-20°C

Notes

Preservative Free
BSA Free

ZO-1 is a peripheral membrane protein that is associated with tight junctions (zonula occludentes) which are found on the cytoplasmic surface of epithelial and endothelial cell membranes. ZO-1 has a MW of 225kD and is found in two isoforms that differ by the presence or absence of an 80 amino acid region known as 'motif-a'. These two isoforms are the basis for determining two classes of tight junctions. ZO-1 is known to interact with ZO-2. ZO-1 is homologous to dlg, the Drosophila tumor suppressor. Both are members of a putative signal transduction protein family whose members contain a SH3 domain and a domain homologous to the enzyme guanylate kinase.

Applications

Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:. Immunohistochemistry: Frozen sections Immunohistochemistry: For formalin fixed, paraffin embedded tissue sections. Protease XIV pretreatment is required Optimal dilutions to be determined by the researcher.

Positive Controls for Immunoprecipitation

Canine MDCK and human Caco-2 cells.

Storage and Stability

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Immunogen

A 69kD fusion protein corresponding to aa463-1109 of human ZO-1 cDNA. This sequence lies from the N-terminal to the 80aa region (the alpha-motif) present in the a+isoform but absent in the alpha- isoform due to alternative splicing.

Form

Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes human ZO-1 alpha+ and ZO-1 alpha- isoforms. Reactivity with dlg has not been characterized. No reactivity with PSD-95 or p55 has been observed on Western Blots, but potential reactivity with these proteins has not been fully characterized. Species Crossreactivity: mouse, rat, guinea pig, canine.

References

1. Willott, E. et al., Am J Physiol. 262 (Cell Physiol. 31):C1119-C1124 (1992). 2. Willott, E. et al., Proc. Natl. Acad. Sci., USA 90(16):7834-7838 (1993). 3. Balda, M.S. and Anderson, J.M., Am J Physiol 264 (Cell Physiol. 33):C918-C924 (1993). 4. Tsarfaty, I, et al., Science 263:98-101 (1994). 5. Keon, BH, et al., J. Cell Biol. 134(4):1003-1018 (1996). 6. Bomsel, M. Nature Medicine 3(1):42-47 (1997). 7. Watabe-Uchida, M, et al., J. Cell Biol 142(3):847-857 (1998). 8. Hoover, KB, et al., J. Am. J. Pathol 153(6):1767-1773 (1998). 9. Rajasekaran, AK, et al., J. Cell Biol 132(3):451-463 (1996). 10. Haskins, J, et al., J. Cell Biol. 141(1):199-208 (1998). 11. Kachinsky, AM, et al., J. Cell Biol. 145:391-402 (1999). 12. Wu, S., et al., J. Proc. Natl. Acad. Sci. 95(25):14979-14984 (1998). 13. Moreira, JE, et al. J. Histochem. Cytochem. 46(7):847-854 (1998).14. Potempa, S. and Ridley, A.J. Mol Biol Cell 9:2185-2200 (1998).15. Mann, B, et al., Proc. Natl. Acad. Sci. 96:1603-1608 (1999).16. Laing, JG, et al., J. Biol. Chem. 276 (25) : 23051-23055 (2001). 17. Hirata, K., et al., J. Biol. Chem. 276 (19) : 16223-16231 (2001).18. Toyofuku, T., et al., J. Biol. Chem. 276 (3) : 1780-1788 (2001).19. Singh, U., et al., J. Biol. Chem. 275 (24): 18407-18417 (2000).20. Bazzoni, G., et al., J. Biol. Chem. 275 (27) : 20520-20526 (2000).21. Fluge, O., et al., World J. Surg. 25: 623-631 (2001).22. Hamazaki, Y., et al., J. Biol. Chem. 277 (1): 455-461 (2002).23. Duffy, H.S., et al., J. Neuroscience 20 :RC114(1-6)

USBio References

No references available

Product	Ext Price
Subtotal:	Subtotal:
Subtotal:	Subtotal:
Total Coupon Savings:	Total Coupon Savings:	()
Your cart is currently empty.
- Coupon Code

Z0750-02B-Biotin Rabbit Anti-ZO-1 (Tight Junction-associated Polypeptide, Zonula Occludens 1) (Biotin)

Clone Type

Host

Source

Conjugate

Isotype

Grade

Applications

Crossreactivity

Shipping Temp

Storage Temp

Notes

References

USBio References

Conjugates

Pricing

Order

Cart Summary

Recently Viewed

Special Offers

Contact Us

Site Map

Technical Resources

Distributors

Payment Methods