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Z0775G Rat Anti-ZO-1 (Tight Junction-associated Polypeptide, Zonula Occludens 1)

Specifications
References
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Swiss Prot
P39447
Isotype
IgG2a,k
Clone Number
10H16
Grade
Affinity Purified
Applications
IHC IP WB
Crossreactivity
Mo
Accession #
NP_033412
Shipping Temp
Blue Ice
Storage Temp
-20°C

Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane associated guanylate kinase (MAGUK) family and include occludin and zona occludin family members ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.

Applications
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 0.5ug/ml detected Z0-1 in 10ug of mouse liver tissue lysate. Optimal dilutions to be determined by the researcher.
Recommended Positive Control
Mouse liver tissue lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified protein corresponding to mouse ZO-1.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse ZO-1, ~220kD.
References
1.D’Angelo Siliciano et al. The journal of Cell Biology 107(6):2389-2399 2. Stevenson B. etal., The Jourmal of Cell Biology 103: 755-766
USBio References
No references available
Conjugates
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