CD34 CAS

Brand

MaxLight™

Swiss Prot

374990, P28906

Grade

Affinity Purified

Gene ID

947

Accession Number

NP_001020280

Molecular Weight

90110

EU Commodity Code

30021010

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

Notes

Preservative Free
BSA Free

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kD, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels >85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.

Applications

Suitable for use in Flow Cytometry, Western Blot and Immunofluorescence. Other applications have not tested.

Recommended Dilution

Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.

Positive Control

KG-1 cells, tonsil, or angiosarcoma

Storage and Stability

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

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138511-AF-ML650 CD34 (Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin) (MaxLight 650) CAS:

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