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138628 CDKN1A, Recombinant, Human, aa1-164, His-Tag (P21, CIP1, SDI1, WAF1, CAP20, CDKN1, MDA-6, p21CIP1) CAS:

Specifications
References
Grade
Purified
Molecular Weight
20.2
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

CDKN1A, also known as P21 or WAF1, plays a critical role in the cellular response to DNA damage, and its overexpression results in cell cycle arrest. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S. However, CDKN1A may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D. This protein may be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage.

Source
Recombinant protein corresponding to aa1-164 from human CDKN1A at N-terminal, fused to His-tag expressed in E.coli.
Molecular Weight
~20.2kD (184aa)
AA Sequence
MGSSHHHHHH SSGLVPRGSH MSEPAGDVRQ NPCGSKACRR LFGPVDSEQL SRDCDALMAG CIQEARERWN FDFVTETPLE GDFAWERVRG LGLPKLYLPT GPRRGRDELG GGRRPGTSPA LLQGTAEEDH VDLSLSCTLV PRSGEQAEGS PGGPGDSQGR KRRQTSMTDF YHSKRRLIFS KRKP
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
~85% (SDS-PAGE)
Concentration
~0.5mg/ml
Form
Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 2M urea, 0.1M sodium chloride, 10% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. EI-Deiry W S., et al. (1994) Cancer Res. 54:1169-1174. 2. Harper J W., et al. (1993) Cell. 75:805-816.
USBio References
No references available
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