Source
Recombinant Mouse from E. coli
Fragment
Ser30~Lys430 (Accession No: P05202)
Sequence
MGHHHHHHSGS-S SWWTHVEMGP PDPILGVTEA FKRDTNSKKM NLGVGAYRDD NGKPYVLPSV RKAEAQIAAK NLDKEYLPIG GLAEFCKASA ELALGENNEV LKSGRFVTVQ TISGTGALRV GASFLQRFFK FSRDVFLPKP SWGNHTPIFR DAGMQLQGYR YYDPKTCGFD FSGALEDISK IPEQSVLLLH ACAHNPTGVD PRPEQWKEIA SVVKKKNLFA FFDMAYQGFA SGDGDKDAWA VRHFIEQGIN VCLCQSYAKN MGLYGERVGA FTVVCKDAEE AKRVESQLKI LIRPLYSNPP LNGARIAATI LTSPDLRKQW LQEVKGMADR IISMRTQLVS NLKKEGSSHN WQHITDQIGM FCFTGLKPEQ VERLTKEFSV YMTKDGRISV AGVTSGNVGY LAHAIHQVTK
Epitope Tag
N-terminal His-Tag
Applications
Suitable for use in ELISA, Western Blot, Immunoprecipitation, SDS-PAGE. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Source
Recombinant, E. coli
Form
Supplied as lyophilized powder from 100mM sodium bicarbonate, 500mM sodium chloride, pH 8.3, 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, 0.05% Proclin 300. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Do not vortex.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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