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168815-AF-PE Kappa Light Chain (HCAK1, Ig Kappa Chain C Region, IGKC, Immunoglobulin KM) (PE) CAS:

Specifications
References
Swiss Prot
P01601 & P01834 (Human)
Grade
Purified
Gene ID
3514 (Human)
Molecular Weight
22.5
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
(HCAK1, Ig Kappa Chain C Region, IGKC, Immunoglobulin KM)

This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Applications
Suitable for use in Flow Cytometry, FLISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution
FLISA: For coating, order Ab without BSA; Flow Cytometry: 0.5-2.0ug/million cells Immunoprecipitation: 1-2ug/500ug protein lysate Western Blot: 0.5-1.0ug/ml; Immunohistochemistry: Frozen & Formalin-fixed: 0.5-1.0ug/ml for 30 min at RT (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes Optimal dilutions to be determined by the researcher.
Positive Control
293T, Raji or hPBL cells. Tonsil or Spleen
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
1. Takahashi H et. al. Pathol Res Prac 189:300-311 (1993). 2. Momose H et. al. Hum Pathol. 23:1115-1119 (1992).
USBio References
No references available
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