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207273 Manumycin A CAS: 52665-74-4

Specifications
References
CAS Number
52665-74-4
Grade
Highly Purified
MDL Number
MFCD00920782
Molecular Formula
C31H38N2O7
Molecular Weight
550.6
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C

Antibiotic. Potent, selective and competitive cell permeable rasfarnesyltransferase inhibitor (IC50= 30nM). Does not affect geranylgeranyltransferase (IC50=180uM). Inhibition is competitive with respect to farnesyl pyrophosphate and non-competitive with respect to Ras. Neutral sphingomyelinase inhibitor. Blocks insulin-induced MAP kinase activation in rat cardiac myocytes (19uM).

CAS No
52665-74-4
Source
Streptomyces parvulus
Formula
C31H38N2O7
Solubility
DMSO or methanol; insoluble in H2O.
Molecular Weight
550.6
Method for Determining Identity
Proton NMR
Purity
≥98% (HPLC)
Form
Supplied as a yellow to brown powder.
Storage and Stability
May be stored at 4°C for short-term only. Long-term storage is recommended at -20°C. Stable for 1 year after receipt. Protect from light when in solution. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards:  All products should be handled by qualified personnel only, trained in laboratory procedures.
References
1. Identification of Rasfarnesyltransferase inhibitors by microbial screening: M. Hara et al.; PNAS 90: 2281 (1993). 2. Inhibitors of Rasfarnesyltransferases: F. Tamanoi; TIBS 18: 349 (1993), (Review). 3. Farnesyltransferase inhibitors: Ras research yields a potential cancer therapeutic: J.B. Gibbs, et al.; Cell 77: 175 (1994). 4. Protein kinase C, but not tyrosine kinases or Ras, plays a critical role in angiotensin II-induced activation of Raf-1 kinase and extracellular signal-regulated protein kinases in cardiac myocytes: Y. Zou et al.; J. Biol. Chem. 271: 33592 (1996). 5. Manumycin inhibits ras signal transduction pathway and induces apoptosis in COLO320-DM human colon tumour cells: A. Di Paolo, et al.; Br. J. Cancer 82: 905 (2000). 6. Manumycin A and its analogues are irreversible inhibitors of neutral sphingomyelinase: C. Arenz, et al.; ChemBioChem 2: 141 (2001). 7. Enhancement of manumycin A-induced apoptosis by methoxyamine in myeloid leukemia cells: M. She, et al.; Leukemia 19: 595 (2005). 8. Binding of manumycin A inhibits IkappaB kinase beta activity: M. Bernier, et al.; J. Biol. Chem. 281: 2551 (2006). 9. A yeast-based genomic strategy highlights the cell protein networks altered by FTase inhibitor peptidomimet- ics: G. Porcu, et al.; Mol. Cancer 9:197 (2010). 10. Targeting farnesyl-transferase as a novel therapeutcstrategy for mevalonate kinase defiency: In vitro and in vivo approaches: L. De Leo, et al.; Pharmacol. Res. 61: 506 (2010)
USBio References
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