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374625 PAT, Recombinant, Streptomyces Viridochromogenes, aa1-183, His-SUMO-Tag (Phosphinothricin N-acetyltransferase) CAS:

Specifications
References
Swiss Prot
Q57146
Grade
Purified
Molecular Weight
36.6
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C
Phosphinothricin-resistance protein

Inactivates phosphinothricin (PPT) by transfer of an acetyl group from acetyl CoA. This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance.

Source
Recombinant protein corresponding to aa1-183 from streptomyces viridochromogenes PAT, fused to His-SUMO-Tag at N-terminal expressed in E. coli.
Molecular Weight
~36.6kD
Amino Acid Sequence
MSPERRPVEIRPATAADMAAVCDIVNHYIETSTVNFRTEPQTPQEWIDDLERLQDRYPWLVAEVEGVVAGIAYAGPWKARNAYDWTVESTVYVSHRHQRLGLGSTLYTHLLKSMEAQGFKSVVAVIGLPNDPSVRLHEALGYTARGTLRAAGYKHGGWHDVGFWQRDFELPAPPRPVRPVTQI
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
Recombinant, E. coli
Purity
~90% (SDS-PAGE)
Form
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. "Cloning of a phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tue494 and its expression in Streptomyces lividans and Escherichia coli." Strauch E., Wohlleben W., Puehler A.Gene 63:65-74(1988).
USBio References
No references available
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