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374922 PTH, Recombinant, E. coli, aa1-194, His-Tag (Peptidyl-tRNA Hydrolase) CAS:

Specifications
References
Swiss Prot
P0A7D1
Grade
Purified
Molecular Weight
25.1
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C
PTH

The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. Involved in lambda inhibition of host protein synthesis. PTH activity may, directly or indirectly, be the target for lambda bar RNA leading to rap cell death.

Source
Recombinant protein corresponding to aa1-194 from E. coli Peptidyl-tRNA Hydrolase, fused to His-Tag at N-terminal expressed in E. coli.
Molecular Weight
~25.1kD
Amino Acid Sequence
MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAKFFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGVAKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQKLIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
Recombinant, E. coli
Purity
≥90% (SDS-PAGE)
Form
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. "Peptidyl-tRNA hydrolase is involved in lambda inhibition of host protein synthesis." Garcia-Villegas M.R., de la Vega F.M., Galindo J.M., Segura M., Buckingham R.H., Guarneros G.EMBO J. 10:3549-3555(1991).
USBio References
No references available
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