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B0807-14X-Biotin Bcl-X (Bcl2 Like 1 Protein, Apoptosis Regulator Bcl-X, Bcl2-L-1, Bcl-2-like Protein 1, BCL2L1, BCLX) (BSA & Azide Free) (Biotin) CAS:

Specifications
Grade
Highly Purified
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free

Bcl-X has 2 isoforms, Bcl-Xl (long) a 241 amino acid protein and Bcl-Xs (short) a 178 amino acid protein lacking a 63 amino acid domain that is well conserved among members of the Bcl-2 family.

Applications
Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipiation and Immunohistochemistry. Not suitable for use in Western Blot. Other applications have not been tested.
Recommended Dilution
Immunoprecipitation (Use Protein A): 2ug/mg protein lysate Immunohistochemistry (formalin/paraffin): 2-4ug/ml for 30min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20min followed by cooling at RT for 20min. Optimal dilutions to be determined by the researcher.
Positive Control
Hodgkin’s lymphoma
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
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