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C2256-65E-ML550 CD5L, CT (CD5 Antigen-like, CD5 Molecule-like, AIM, API6, CT-2, IgM-associated Peptide, PRO229, SPalpha, SP-alpha) (MaxLight 550) CAS:

Specifications
References
Brand
MaxLight™
Swiss Prot
O43866
Grade
Affinity Purified
Gene ID
922
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
CD5 Antigen-like (Scavenger Receptor Cysteine-rich Family)

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

CD5L has been shown to mediate the survival of macrophages in atherosclerotic plaques.
Applications
Suitable for use in FLISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: detects a band of ~38kD in Raji cell lysates. Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer, pH 6.0 is recommended. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
1. Sarrias, M.R. et al. (2005) A role for human Sp alpha as a pattern recognition receptor. J. Biol. Chem. 280: 35391-35398. 2. Arai, S. et al. (2005) A role for the apoptosis inhibitory factor AIM/Spalpha/Api6 in atherosclerosis development. Cell Metab. 1: 201-213.
USBio References
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