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C2409-14B-ML550 CD54 (ICAM-1, Intercellular Adhesion Molecule 1, Human Rhinovirus Receptor, Precursor, BB2, Major Group Rhinovirus Receptor, P3.58) (MaxLight 550) CAS:

Specifications
References
Brand
MaxLight™
Swiss Prot
P05362
Grade
Affinity Purified
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

CD54 permits antigen-independent adhesion between lymphocytes and their targets and may be used to study cell-cell interactionsor for rhinovirus and malarial studies. The LFA-1 binding site is located in domain 1 of ICAM-1. CD54 antigen is a membrane glycoprotein of ~90kD with a wide tissue distribution that includes vascular endothelium and many cells of the immune system. CD54 is weakly expressed on resting peripheral blood lymphocytes. Upon activation by mitogens, the CD54 antigen is strongly expressed on B-cells, T-cells, macrophages and granulocytes.
Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Frozen and paraffin nsections Optimal working dilutions to be determined by researcher.
Recommended Positive Control
Human Tonsil
Hybridoma
Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. 
Note: Applications are based on unconjugated antibody.
References
1. J. Cell Biol. (1992) 116: 1527-1535. 2. Cell (1992) 68: 71-81. 3. Treadwell, M.D. et al. (1996). Increased neutrophil adherence to endothelial cells exposed to asbestos. Toxicol Appl. Pharmacol. 139: 62-70.
USBio References
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