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C2548-80H-ML650 CD147 (Extracellular Matrix Metalloproteinase Inducer, Basigin, Neurothelin, 5A11 antigen, 5F7, Blood-brain barrier HT7 antigen, Collagenase stimulatory factor, Emmprin, Leukocyte activation antigen M6, M6, OK, OK blood group antigen, TCSF, Tumor cell derived collagenase stimulatory factor) (MaxLight 650) CAS:

Specifications
References
Brand
MaxLight™
Swiss Prot
P18572
Grade
Affinity Purified
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

CD147 (50-60kD) has a wide tissue distribution on hematopoietic and non-hematopoietic cells including leukocytes, red blood cells, platelets and endothelial cells. As a potential adhesion molecule, CD147 may be involved in embryonic blood-brain barrier development and in integrin-mediated adhesion in brain endothelia. The reported functions of CD147 are diverse and include involvement in T-cell activation, cell adhesion and induction of matrix metalloproteinases.
Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
Immunohistochemstry: Frozen sections Optimal dilutions to be determined by the researcher.
Recommended Negative Control
I1904-76H: Rat IgG1 Negative Control
Recommended Secondary Reagents
I1904-47Y: IgG, Fc (HRP) Pab Gt xRt I1904-47T: IgG, H&L (HRP) Pab Gt xRt I1904-47N: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRt I1904-47H: IgG, H&L (HRP) Pab Gt xRt I1904-50U1: IgG H&L, X-adsorbed (HRP) Pab Gt xRt
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. 
Note: Applications are based on unconjugated antibody.
References
1. Hanna, S.M., et al., BMC Biochemistry 4: 17 (2003). 2. Agrawal S.M. et al., Journal of Neurosci. 31:669-77 (2011). 3. Sweigard, J.H. et al., |Gene Ther. 18 6:613-21 (2011)
USBio References
No references available
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