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C2589-94 CEBP gamma, DNA Binding Domain, Recombinant, Human, aa39-147, His-Tag (CCAAT Enhancer Binding Protein, gamma) CAS:

Specifications
References
Swiss Prot
P53567
Grade
Highly Purified
Accession Number
NP_001797
Molecular Weight
16.5
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

CCAAT/enhancer binding protein (C/EBP) gamma is a family of transcription factors all contain a highly conserved, basic-leucine zipper domain at the C-terminus that is involved in dimerization and DNA binding. C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP family consist of several related proteins, C/EBP alpha, beta, gamma, delta, that form homodimers and/or form heterodimers with each other. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein; a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. C/EBP gamma may cooperate with Fos to bind PRE-enhancer elements.

The DNA binding domain of CEBP gamma (amino acid residues, 39-147) was produced in E .coli and purified by using conventional chromatography techniques.
Molecular Weight
16.5kD (146 amino acids)
Sequence
MRGSHHHHHH/ GMASMTGGQQ/ MGRDLYDDDD/ KDRWGSMPGG/ GGKAVAPSKQ/ SKKSSPMDRN/ SDEYRQRRER/ NNMAVKKSRL/ KSKQKAQDTL/ QRVNQLKEEN/ ERLEAKIKLL/ TKELSVLKDL/ FLEHAHNLAD/ NVQSISTENT/ TADGDN
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
Purified by conventional chromatography techniques (≥ 95% by SDS PAGE).
Concentration
~1mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 0.1M sodium chloride, 5mM beta-Mercaptoethanol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Hattori, T., et al., Oncogene 22(9): 1273-1280 (2003). 2. Davydov, I., et al., Gene 161(2): 271-275 (1995). 3. Williams, S.C., et al., Genes Dev. 5(9): 1553-1567 (1991).
USBio References
No references available
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