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C7915-27-ML650 CREB (cAMP Response Element Binding Protein) (MaxLight 650) CAS:

Specifications
References
Brand
MaxLight™
Grade
Affinity Purified
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Transcription factor CREB binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth or neurotrophic factors. Protein kinase A and the calmodulin-dependent protein kinase CaMKII stimulate CREB phosphorylation at Ser133, a key regulatory site controlling transcriptional activity (1,2). Phosphorylation at Ser133 is also mediated by p44/42 MAP kinase, p90RSK, p38 MAP kinase and MSK1 (3–5). CREB appears to play an important role in learning and memory in both flies and mice.
Applications
Suitable for use in FLISA and Western Blot. Other applications not tested.
Recommended Dilutions
FLISA: 0.1-1ug/ml Western Blot: 1-5ug/ml Optimal dilutions to be determined by the researcher.
Positive Controls
Human H9 and Jurkat lymphoblasts, HeLa cells, Hep G2 hepatoma cells and WI-38 fibroblasts, rat 208F, mouse BALB/c 3T3 fibroblasts and F9 embryonal cell carcinoma stem cells.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
Laura A. Berkowitz and Michael Z. Gilman. Two distinct forms of active transcription factor CREB (cAMP response element binding protein). PNAS 87: 5258–5262, (1990).
USBio References
No references available
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