DnaK, Recombinant, E. coli, aa508-638 CAS

Grade

Highly Purified

Molecular Weight

14.6

EU Commodity Code

30021019

Shipping Temp

Blue Ice

Storage Temp

-20°C

DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins.

DnaK (residues 508-638) of the substrate binding domain is alpha helical and appears to act as a lid covering the substrate binding cleft. Additional amino acid (Met) is attached at the N-terminus.

Molecular Weight

14.6kD (132 amino acids)

Sequence

MNEDEIQKMV RDAEANAEAD RKFEELVQTR NQGDHLLHST RKQVEEAGDK LPADDKTAIESALTALETAL KGEDKAAIEA KMQELAQVSQ KLMEIAQQQH AQQQTAGADA SANNAKDDDVVDAEFEEVKDKK

Biological Activity: Not determined

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Source

Recombinant, E. coli

Purity

≥ 95% by SDS PAGE; Purified by using conventional chromatography techniques.

Concentration

~1mg/ml

Form

Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 100mM sodium chloride, 1mM DTT, 10% glycerol.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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D4015-14A DnaK, Recombinant, E. coli, aa508-638 CAS:

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